cd56 conjugated primary antibody 130 128 471 Search Results


apc vio770 conjugated anticd56  (Miltenyi Biotec)
94
Miltenyi Biotec apc vio770 conjugated anticd56
Apc Vio770 Conjugated Anticd56, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd19  (Miltenyi Biotec)
96
Miltenyi Biotec cd19
SMN levels were analyzed by CD+ cell subtype at the 7d timepoint from Study 4. A : Analysis of SMN by PBMC cell subpopulation revealed that the CD14+ population had statistically significant reductions in SMN levels. B : Fractionation of PBMCs with normalization by cell count showed no differences in SMN signal in group analysis. C : Evaluation of total soluble protein levels by CD+ population revealed that CD14+ cells had double the protein concentrations of CD4+, CD8+, <t>CD19+,</t> and CD56+ cells. This differential is sufficient to drive variability in situations that cause CD14+ populations to fluctuate. D : SMN levels (normalized by protein) show consistently lower levels in CD14+ fractions compared to all other fractions, with differences up to 7x within individual PBMC subpopulations. E : SMN in individuals as measured by cell counts were also variable ranging up to 3.5x between individuals’ subcellular populations, but was overall less variable than protein normalized SMN measures. In the bodies of the boxplots indicate the first and third quartiles, while the horizontal bar indicates the median. In error bars depict standard deviation.
Cd19, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd3 pacific blue  (Becton Dickinson)
90
Becton Dickinson anti cd3 pacific blue
SMN levels were analyzed by CD+ cell subtype at the 7d timepoint from Study 4. A : Analysis of SMN by PBMC cell subpopulation revealed that the CD14+ population had statistically significant reductions in SMN levels. B : Fractionation of PBMCs with normalization by cell count showed no differences in SMN signal in group analysis. C : Evaluation of total soluble protein levels by CD+ population revealed that CD14+ cells had double the protein concentrations of CD4+, CD8+, <t>CD19+,</t> and CD56+ cells. This differential is sufficient to drive variability in situations that cause CD14+ populations to fluctuate. D : SMN levels (normalized by protein) show consistently lower levels in CD14+ fractions compared to all other fractions, with differences up to 7x within individual PBMC subpopulations. E : SMN in individuals as measured by cell counts were also variable ranging up to 3.5x between individuals’ subcellular populations, but was overall less variable than protein normalized SMN measures. In the bodies of the boxplots indicate the first and third quartiles, while the horizontal bar indicates the median. In error bars depict standard deviation.
Anti Cd3 Pacific Blue, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ccr4 pe rea279 miltenyi  (Miltenyi Biotec)
93
Miltenyi Biotec ccr4 pe rea279 miltenyi
SMN levels were analyzed by CD+ cell subtype at the 7d timepoint from Study 4. A : Analysis of SMN by PBMC cell subpopulation revealed that the CD14+ population had statistically significant reductions in SMN levels. B : Fractionation of PBMCs with normalization by cell count showed no differences in SMN signal in group analysis. C : Evaluation of total soluble protein levels by CD+ population revealed that CD14+ cells had double the protein concentrations of CD4+, CD8+, <t>CD19+,</t> and CD56+ cells. This differential is sufficient to drive variability in situations that cause CD14+ populations to fluctuate. D : SMN levels (normalized by protein) show consistently lower levels in CD14+ fractions compared to all other fractions, with differences up to 7x within individual PBMC subpopulations. E : SMN in individuals as measured by cell counts were also variable ranging up to 3.5x between individuals’ subcellular populations, but was overall less variable than protein normalized SMN measures. In the bodies of the boxplots indicate the first and third quartiles, while the horizontal bar indicates the median. In error bars depict standard deviation.
Ccr4 Pe Rea279 Miltenyi, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti human cd56 microbeads  (Miltenyi Biotec)
96
Miltenyi Biotec anti human cd56 microbeads
SMN levels were analyzed by CD+ cell subtype at the 7d timepoint from Study 4. A : Analysis of SMN by PBMC cell subpopulation revealed that the CD14+ population had statistically significant reductions in SMN levels. B : Fractionation of PBMCs with normalization by cell count showed no differences in SMN signal in group analysis. C : Evaluation of total soluble protein levels by CD+ population revealed that CD14+ cells had double the protein concentrations of CD4+, CD8+, <t>CD19+,</t> and CD56+ cells. This differential is sufficient to drive variability in situations that cause CD14+ populations to fluctuate. D : SMN levels (normalized by protein) show consistently lower levels in CD14+ fractions compared to all other fractions, with differences up to 7x within individual PBMC subpopulations. E : SMN in individuals as measured by cell counts were also variable ranging up to 3.5x between individuals’ subcellular populations, but was overall less variable than protein normalized SMN measures. In the bodies of the boxplots indicate the first and third quartiles, while the horizontal bar indicates the median. In error bars depict standard deviation.
Anti Human Cd56 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat anti mouse igm  (Miltenyi Biotec)
96
Miltenyi Biotec rat anti mouse igm
SMN levels were analyzed by CD+ cell subtype at the 7d timepoint from Study 4. A : Analysis of SMN by PBMC cell subpopulation revealed that the CD14+ population had statistically significant reductions in SMN levels. B : Fractionation of PBMCs with normalization by cell count showed no differences in SMN signal in group analysis. C : Evaluation of total soluble protein levels by CD+ population revealed that CD14+ cells had double the protein concentrations of CD4+, CD8+, <t>CD19+,</t> and CD56+ cells. This differential is sufficient to drive variability in situations that cause CD14+ populations to fluctuate. D : SMN levels (normalized by protein) show consistently lower levels in CD14+ fractions compared to all other fractions, with differences up to 7x within individual PBMC subpopulations. E : SMN in individuals as measured by cell counts were also variable ranging up to 3.5x between individuals’ subcellular populations, but was overall less variable than protein normalized SMN measures. In the bodies of the boxplots indicate the first and third quartiles, while the horizontal bar indicates the median. In error bars depict standard deviation.
Rat Anti Mouse Igm, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd4 t cell isolation  (Miltenyi Biotec)
97
Miltenyi Biotec cd4 t cell isolation
SMN levels were analyzed by CD+ cell subtype at the 7d timepoint from Study 4. A : Analysis of SMN by PBMC cell subpopulation revealed that the CD14+ population had statistically significant reductions in SMN levels. B : Fractionation of PBMCs with normalization by cell count showed no differences in SMN signal in group analysis. C : Evaluation of total soluble protein levels by CD+ population revealed that CD14+ cells had double the protein concentrations of CD4+, CD8+, <t>CD19+,</t> and CD56+ cells. This differential is sufficient to drive variability in situations that cause CD14+ populations to fluctuate. D : SMN levels (normalized by protein) show consistently lower levels in CD14+ fractions compared to all other fractions, with differences up to 7x within individual PBMC subpopulations. E : SMN in individuals as measured by cell counts were also variable ranging up to 3.5x between individuals’ subcellular populations, but was overall less variable than protein normalized SMN measures. In the bodies of the boxplots indicate the first and third quartiles, while the horizontal bar indicates the median. In error bars depict standard deviation.
Cd4 T Cell Isolation, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd56 ncam  (Miltenyi Biotec)
93
Miltenyi Biotec cd56 ncam
SMN levels were analyzed by CD+ cell subtype at the 7d timepoint from Study 4. A : Analysis of SMN by PBMC cell subpopulation revealed that the CD14+ population had statistically significant reductions in SMN levels. B : Fractionation of PBMCs with normalization by cell count showed no differences in SMN signal in group analysis. C : Evaluation of total soluble protein levels by CD+ population revealed that CD14+ cells had double the protein concentrations of CD4+, CD8+, <t>CD19+,</t> and CD56+ cells. This differential is sufficient to drive variability in situations that cause CD14+ populations to fluctuate. D : SMN levels (normalized by protein) show consistently lower levels in CD14+ fractions compared to all other fractions, with differences up to 7x within individual PBMC subpopulations. E : SMN in individuals as measured by cell counts were also variable ranging up to 3.5x between individuals’ subcellular populations, but was overall less variable than protein normalized SMN measures. In the bodies of the boxplots indicate the first and third quartiles, while the horizontal bar indicates the median. In error bars depict standard deviation.
Cd56 Ncam, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd49b rea188 apc miltenyi biotec  (Miltenyi Biotec)
96
Miltenyi Biotec cd49b rea188 apc miltenyi biotec
SMN levels were analyzed by CD+ cell subtype at the 7d timepoint from Study 4. A : Analysis of SMN by PBMC cell subpopulation revealed that the CD14+ population had statistically significant reductions in SMN levels. B : Fractionation of PBMCs with normalization by cell count showed no differences in SMN signal in group analysis. C : Evaluation of total soluble protein levels by CD+ population revealed that CD14+ cells had double the protein concentrations of CD4+, CD8+, <t>CD19+,</t> and CD56+ cells. This differential is sufficient to drive variability in situations that cause CD14+ populations to fluctuate. D : SMN levels (normalized by protein) show consistently lower levels in CD14+ fractions compared to all other fractions, with differences up to 7x within individual PBMC subpopulations. E : SMN in individuals as measured by cell counts were also variable ranging up to 3.5x between individuals’ subcellular populations, but was overall less variable than protein normalized SMN measures. In the bodies of the boxplots indicate the first and third quartiles, while the horizontal bar indicates the median. In error bars depict standard deviation.
Cd49b Rea188 Apc Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotin antibody cocktail  (Miltenyi Biotec)
96
Miltenyi Biotec biotin antibody cocktail
SMN levels were analyzed by CD+ cell subtype at the 7d timepoint from Study 4. A : Analysis of SMN by PBMC cell subpopulation revealed that the CD14+ population had statistically significant reductions in SMN levels. B : Fractionation of PBMCs with normalization by cell count showed no differences in SMN signal in group analysis. C : Evaluation of total soluble protein levels by CD+ population revealed that CD14+ cells had double the protein concentrations of CD4+, CD8+, <t>CD19+,</t> and CD56+ cells. This differential is sufficient to drive variability in situations that cause CD14+ populations to fluctuate. D : SMN levels (normalized by protein) show consistently lower levels in CD14+ fractions compared to all other fractions, with differences up to 7x within individual PBMC subpopulations. E : SMN in individuals as measured by cell counts were also variable ranging up to 3.5x between individuals’ subcellular populations, but was overall less variable than protein normalized SMN measures. In the bodies of the boxplots indicate the first and third quartiles, while the horizontal bar indicates the median. In error bars depict standard deviation.
Biotin Antibody Cocktail, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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realease cd56 pe releasable antibodies  (Miltenyi Biotec)
92
Miltenyi Biotec realease cd56 pe releasable antibodies
SMN levels were analyzed by CD+ cell subtype at the 7d timepoint from Study 4. A : Analysis of SMN by PBMC cell subpopulation revealed that the CD14+ population had statistically significant reductions in SMN levels. B : Fractionation of PBMCs with normalization by cell count showed no differences in SMN signal in group analysis. C : Evaluation of total soluble protein levels by CD+ population revealed that CD14+ cells had double the protein concentrations of CD4+, CD8+, <t>CD19+,</t> and CD56+ cells. This differential is sufficient to drive variability in situations that cause CD14+ populations to fluctuate. D : SMN levels (normalized by protein) show consistently lower levels in CD14+ fractions compared to all other fractions, with differences up to 7x within individual PBMC subpopulations. E : SMN in individuals as measured by cell counts were also variable ranging up to 3.5x between individuals’ subcellular populations, but was overall less variable than protein normalized SMN measures. In the bodies of the boxplots indicate the first and third quartiles, while the horizontal bar indicates the median. In error bars depict standard deviation.
Realease Cd56 Pe Releasable Antibodies, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd8a fab clone bw135 80  (Miltenyi Biotec)
95
Miltenyi Biotec anti cd8a fab clone bw135 80
SMN levels were analyzed by CD+ cell subtype at the 7d timepoint from Study 4. A : Analysis of SMN by PBMC cell subpopulation revealed that the CD14+ population had statistically significant reductions in SMN levels. B : Fractionation of PBMCs with normalization by cell count showed no differences in SMN signal in group analysis. C : Evaluation of total soluble protein levels by CD+ population revealed that CD14+ cells had double the protein concentrations of CD4+, CD8+, <t>CD19+,</t> and CD56+ cells. This differential is sufficient to drive variability in situations that cause CD14+ populations to fluctuate. D : SMN levels (normalized by protein) show consistently lower levels in CD14+ fractions compared to all other fractions, with differences up to 7x within individual PBMC subpopulations. E : SMN in individuals as measured by cell counts were also variable ranging up to 3.5x between individuals’ subcellular populations, but was overall less variable than protein normalized SMN measures. In the bodies of the boxplots indicate the first and third quartiles, while the horizontal bar indicates the median. In error bars depict standard deviation.
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SMN levels were analyzed by CD+ cell subtype at the 7d timepoint from Study 4. A : Analysis of SMN by PBMC cell subpopulation revealed that the CD14+ population had statistically significant reductions in SMN levels. B : Fractionation of PBMCs with normalization by cell count showed no differences in SMN signal in group analysis. C : Evaluation of total soluble protein levels by CD+ population revealed that CD14+ cells had double the protein concentrations of CD4+, CD8+, CD19+, and CD56+ cells. This differential is sufficient to drive variability in situations that cause CD14+ populations to fluctuate. D : SMN levels (normalized by protein) show consistently lower levels in CD14+ fractions compared to all other fractions, with differences up to 7x within individual PBMC subpopulations. E : SMN in individuals as measured by cell counts were also variable ranging up to 3.5x between individuals’ subcellular populations, but was overall less variable than protein normalized SMN measures. In the bodies of the boxplots indicate the first and third quartiles, while the horizontal bar indicates the median. In error bars depict standard deviation.

Journal: PLoS ONE

Article Title: Evaluation of Peripheral Blood Mononuclear Cell Processing and Analysis for Survival Motor Neuron Protein

doi: 10.1371/journal.pone.0050763

Figure Lengend Snippet: SMN levels were analyzed by CD+ cell subtype at the 7d timepoint from Study 4. A : Analysis of SMN by PBMC cell subpopulation revealed that the CD14+ population had statistically significant reductions in SMN levels. B : Fractionation of PBMCs with normalization by cell count showed no differences in SMN signal in group analysis. C : Evaluation of total soluble protein levels by CD+ population revealed that CD14+ cells had double the protein concentrations of CD4+, CD8+, CD19+, and CD56+ cells. This differential is sufficient to drive variability in situations that cause CD14+ populations to fluctuate. D : SMN levels (normalized by protein) show consistently lower levels in CD14+ fractions compared to all other fractions, with differences up to 7x within individual PBMC subpopulations. E : SMN in individuals as measured by cell counts were also variable ranging up to 3.5x between individuals’ subcellular populations, but was overall less variable than protein normalized SMN measures. In the bodies of the boxplots indicate the first and third quartiles, while the horizontal bar indicates the median. In error bars depict standard deviation.

Article Snippet: The order of addition of magnetic beads with antibodies for positive selection of PBMC subsets were CD14, CD56, CD19, CD8, and CD4 (Miltenyi OctoMACs #130-050-201, #130-050-401, #130-050-301, #130-045-201, #130-045-101 respectively).

Techniques: Fractionation, Cell Counting, Standard Deviation